| Gene name |
SPBC11B10.10c |
| Gene ID |
41/D01 |
| Gene synonyms/obsolete |
pht1; pi001 |
| Gene product |
histone h2a variant;
involved in chromosome stability; involved in chromatin
assembly/disassembly; chromatin assembly complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
516 |
| ORF length (spliced) |
|
| Entry clone length |
516 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
343T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC11B10.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATATTAAGACACGCACC |
| Rev primer name |
SPBC11B10.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATAATTTCTTCCTCCTCG |
| Amino acid length |
171 |
| Molecular weight |
18.8 |
| Isoelectric point (calc.) |
10.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPHINKQLLI |
| Localization (YFP) |
nucleus;
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |