Gene name |
SPBP22H7.03 |
Gene ID |
41/D02 |
Gene synonyms/obsolete |
pi028;
SPACTOKYO_453.06c; SPACTOKYO_453.07 |
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
546 |
ORF length (spliced) |
|
Entry clone length |
546 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
194A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP22H7.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCAGTCACCTATAGA |
Rev primer name |
SPBP22H7.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTTTTTCTTCCTTTGCTTT |
Amino acid length |
181 |
Molecular weight |
20.2 |
Isoelectric point (calc.) |
3.7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRGFVNILTL/LSCGLLMLFI |
Localization (YFP) |
cytoplasmic dots,
especially at cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |