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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G7.09c
Gene ID 42/A12
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 408
ORF length (spliced)
Entry clone length 408
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC13G7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAATCATCACTTGACAG
Rev primer name SPAC13G7.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTTTCTCAATTTTTAAT
Amino acid length 135
Molecular weight 16
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 66
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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