| Gene name |
SPCC613.05c |
| Gene ID |
42/F12 |
| Gene synonyms/obsolete |
rpl35 |
| Gene product |
60S ribosomal protein
L35 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
653 |
| ORF length (spliced) |
369 |
| Entry clone length |
653 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC613.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTAAAGACCTTTGA |
| Rev primer name |
SPCC613.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCCTTAAGAGCATACTTG |
| Amino acid length |
122 |
| Molecular weight |
14.3 |
| Isoelectric point (calc.) |
11.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; occasionally
nucleolus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |