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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4G9.11c
Gene ID 42/H05
Gene synonyms/obsolete cmb1
Gene product HMG box mismatch binding protein vHMG box; involved in DNA repair; involved in mismatch repair; binds to cytosines in base mismatches and opposite chemically altered guanines
Entry clone Cloned
ORF length (unspliced) 672
ORF length (spliced)
Entry clone length 672
No. of intron 0
Sequence status Finished
Sequence results 4C:T
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC4G9.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGTCTGTTTGACGCAAT
Rev primer name SPAC4G9.11.Rv
Rev primer SEQ AGAAAGCTGGGTATCGAAATCCGGCTTCTTTC
Amino acid length 223
Molecular weight 26.3
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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