Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC13G1.07
Gene ID 43/G02
Gene synonyms/obsolete
Gene product zinc finger protein; zf-DHHC type; palmitoyltransferase activity; involved in palmitoylation
Entry clone Cloned
ORF length (unspliced) 1071
ORF length (spliced)
Entry clone length 1071
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC13G1.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTCTTTTATAAATA
Rev primer name SPBC13G1.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTTGTTGAATAAAGGTGG
Amino acid length 356
Molecular weight 41.4
Isoelectric point (calc.) 9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 5
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVVFLYLAL/LISLICSVLVL
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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