Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC30.12
Gene ID 43/G03
Gene synonyms/obsolete tbp1; tdf1; tbp; SPAC29E6.08
Gene product TATA-binding protein TBP; transcription initiation factor TFIID subunit
Entry clone Cloned
ORF length (unspliced) 1073
ORF length (spliced) 696
Entry clone length 1073
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC30.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTCGCTTTACCCAC
Rev primer name SPAC30.12.Rv
Rev primer SEQ AGAAAGCTGGGTAATGTTTTCGAAATTCAGAC
Amino acid length 231
Molecular weight 25.4
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.