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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP27G11.08c
Gene ID 44/A09
Gene synonyms/obsolete
Gene product hypothetical proteinhypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1229
ORF length (spliced) 1179
Entry clone length 1229
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAP27G11.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGTTTACTTATCTGA
Rev primer name SPAP27G11.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAATGTATGATTTTAAGGGT
Amino acid length 392
Molecular weight 44.5
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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