| Gene name |
SPAC144.14 |
| Gene ID |
45/C05 |
| Gene synonyms/obsolete |
klp8 |
| Gene product |
kinesin-like
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1588 |
| ORF length (spliced) |
1536 |
| Entry clone length |
1588 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC144.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTACAGCTAATGTTCG |
| Rev primer name |
SPAC144.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTAACAAGGAATCATGC |
| Amino acid length |
511 |
| Molecular weight |
57.2 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSSLFKDLKL |
| Localization (YFP) |
SPB; nuclear dots;
periphery at cell tip and site of septum formation;
cytoplasmic microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |