| Gene name |
SPAC13G6.10c |
| Gene ID |
45/C06 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; serine-rich protein; similar to N. crassa
B24p11.210; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1593 |
| ORF length (spliced) |
|
| Entry clone length |
1593 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1364G:T |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC13G6.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTACTACTTTCGCGAC |
| Rev primer name |
SPAC13G6.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAAATATAAATCTCTCCG |
| Amino acid length |
530 |
| Molecular weight |
54.2 |
| Isoelectric point (calc.) |
3.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER; Golgi? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |