Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC17D1.08
Gene ID 45/E08
Gene synonyms/obsolete SPBC11C11.01
Gene product conserved hypothetical; hypothetical protein; rrm RNA recognition motif; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1676
ORF length (spliced) 1482
Entry clone length 1676
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC17D1.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAAAAATAATGAAAG
Rev primer name SPBC17D1.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGAATAAATCCTCCGCATGC
Amino acid length 493
Molecular weight 57.1
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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