Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16G5.17
Gene ID 45/E09
Gene synonyms/obsolete
Gene product transcriptional regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain; similar to Sp SPAC3H8.08C
Entry clone Cloned
ORF length (unspliced) 1683
ORF length (spliced)
Entry clone length 1683
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC16G5.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTGGGAAATCTAAAAA
Rev primer name SPBC16G5.17.Rv
Rev primer SEQ AGAAAGCTGGGTATAAGTTTGGTAATGGAAAA
Amino acid length 560
Molecular weight 65.1
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGQLLTCGLEI
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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