| Gene name |
SPCC576.17c |
| Gene ID |
45/G08 |
| Gene synonyms/obsolete |
|
| Gene product |
transporter; unknown
specificity; similar to Sp car1 (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1753 |
| ORF length (spliced) |
1578 |
| Entry clone length |
1753 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC576.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGATACAAACGATGT |
| Rev primer name |
SPCC576.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGGGCAGCCGTGTTGATT |
| Amino acid length |
525 |
| Molecular weight |
59.3 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
13 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGVALGPLFL/LVYIGSLII |
| Localization (YFP) |
periphery; cytoplasmic
dots |
| Comments for localization |
ambiguous
structure |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |