| Gene name |
SPCC1259.13 |
| Gene ID |
45/G09 |
| Gene synonyms/obsolete |
chk1; rad27 |
| Gene product |
serine/threonine
protein kinase; involved in DNA repair; involved in DNA damage
checkpoint; interacts physically with Ded1p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1756 |
| ORF length (spliced) |
1491 |
| Entry clone length |
1756 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1259.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTCAAAAATTAGATAA |
| Rev primer name |
SPCC1259.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTGTGAAACATCTGTA |
| Amino acid length |
496 |
| Molecular weight |
56.5 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LASRLMLKLRI/LYDSLRLLAI/LTNLGHNLEL |
| Localization (YFP) |
nucleus>cytosol;
cytoplasmic dots; nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |