Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A7.13
Gene ID 46/A06
Gene synonyms/obsolete
Gene product RNA-binding protein; rrm RNA recognition motif (2); G-patch domain; zinc finger protein; zf-C2H2 type; similar to human tumor suppressor luca15; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1865
ORF length (spliced) 1698
Entry clone length 1865
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC57A7.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGGATTATTATTCAGA
Rev primer name SPAC57A7.13.Rv
Rev primer SEQ AGAAAGCTGGGTATAAATTATTGTTTTTATCA
Amino acid length 565
Molecular weight 64.4
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LWKGLSKLEI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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