| Gene name |
SPAC23D3.10c |
| Gene ID |
46/E09 |
| Gene synonyms/obsolete |
eng2 |
| Gene product |
glycosyl hydrolase
family 81; endo-1,3-beta-glucanase; similar to Sp eng1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2121 |
| ORF length (spliced) |
|
| Entry clone length |
2121 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC23D3.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTTTTAGTACCAAT |
| Rev primer name |
SPAC23D3.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGGTGATCCACCCATACCA |
| Amino acid length |
706 |
| Molecular weight |
77.9 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPTQVGFLPI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |