| Gene name |
SPAC3H1.09c |
| Gene ID |
46/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
amino acid transporter
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
2123 |
| ORF length (spliced) |
1971 |
| Entry clone length |
2123 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
1894T:addition |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC3H1.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAATAGTCAGTCTAT |
| Rev primer name |
SPAC3H1.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAAATGTCATGTACGTA |
| Amino acid length |
656 |
| Molecular weight |
73 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
568 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSHICFLLLI/LIADVFILLGI/LRVLIVILAI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |