Gene name |
SPAC4G8.04 |
Gene ID |
46/H01 |
Gene synonyms/obsolete |
|
Gene product |
TBC domain protein;
GTPase activating protein; Rab-like GTPase |
Entry clone |
Cloned |
ORF length (unspliced) |
2382 |
ORF length (spliced) |
2319 |
Entry clone length |
2382 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
2094T:deletion |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC4G8.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAACCATCTGTTTCTGA |
Rev primer name |
SPAC4G8.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAATTGACCGCTAAGTTT |
Amino acid length |
772 |
Molecular weight |
86.2 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHSIPGSLHL/LPEIYSHLEL |
Localization (YFP) |
periphery with
discontinuity |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |