| Gene name |
SPBC354.08c |
| Gene ID |
47/A04 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; DUF221; involved in
sporulation; non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2634 |
| ORF length (spliced) |
2598 |
| Entry clone length |
2634 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC354.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAATTGAGAAACGTGA |
| Rev primer name |
SPBC354.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAATGTGGTAATATTTA |
| Amino acid length |
865 |
| Molecular weight |
99.2 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSVILLCI/LANIILFLCL |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |