| Gene name |
SPAC22F8.11 |
| Gene ID |
47/B02 |
| Gene synonyms/obsolete |
plc1 |
| Gene product |
1-phosphatidylinositol-4,5-bisphosphate
phosphodiesterase 1; phosphoinositide-specific phospholipase
C; EF hand motifs; calcium binding protein; C2 domain;
pleckstrin homology domain; complexed with 14-3-3 proteins;
involved in UV protection |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2700 |
| ORF length (spliced) |
|
| Entry clone length |
2700 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC22F8.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTGTGAAATGGTAAC |
| Rev primer name |
SPAC22F8.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGTCTTCTTTTTTAATT |
| Amino acid length |
899 |
| Molecular weight |
102.6 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNWLSLKLNL/LDPISGYLML/LNEFIIGLNL |
| Localization (YFP) |
cytosol=nucleus;
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |