| Gene name |
SPAC22A12.07c |
| Gene ID |
47/B01 |
| Gene synonyms/obsolete |
|
| Gene product |
dolichyl-phosphate-mannose-protein
mannosyltransferase; MIR domains |
| Entry clone |
Cloned directly into
pDUAL-FFH1 |
| ORF length (unspliced) |
2682 |
| ORF length (spliced) |
|
| Entry clone length |
2682 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC22A12.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAAACAATCCACTTT |
| Rev primer name |
SPAC22A12.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCCACACGAGCTTGAGCA |
| Amino acid length |
893 |
| Molecular weight |
101.3 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTGVALGLAL |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|