Gene name |
SPCC1322.01 |
Gene ID |
47/D03 |
Gene synonyms/obsolete |
SPCC23B6.06 |
Gene product |
3'-5' exonuclease for
RNA 3' ss-tail; involved in mitochondrial biogenesis |
Entry clone |
Cloned |
ORF length (unspliced) |
2874 |
ORF length (spliced) |
|
Entry clone length |
2874 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1322.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACTATAGGCAATTGTT |
Rev primer name |
SPCC1322.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTCGCTAAAGCAACTAAT |
Amino acid length |
957 |
Molecular weight |
109.9 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLKRVCLSLKL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |