Gene name |
SPCC18B5.08c |
Gene ID |
47/E04 |
Gene synonyms/obsolete |
|
Gene product |
isoleucine-tRNA
ligase; involved in isoleucyl-tRNA aminoacylation;
isoleucine-tRNA ligase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2922 |
ORF length (spliced) |
|
Entry clone length |
2922 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC18B5.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATTTTCATATATAAG |
Rev primer name |
SPCC18B5.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCGTTTCAGTTGGGCCAAA |
Amino acid length |
973 |
Molecular weight |
111.2 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPYGVDLLRL/LRGIDKLAL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |