Gene name |
SPAC1327.01c |
Gene ID |
47/E07 |
Gene synonyms/obsolete |
SPAC18G6.16c;
SPAC1783.09c |
Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain |
Entry clone |
Cloned |
ORF length (unspliced) |
2934 |
ORF length (spliced) |
|
Entry clone length |
2934 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1327.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGCAACAGCGTTCAGG |
Rev primer name |
SPAC1327.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCGGCAAACGAAATATTC |
Amino acid length |
977 |
Molecular weight |
107.8 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
64 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nuclear dots; nucleus;
cytoplasmic dots |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |