Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1327.01c
Gene ID 47/E07
Gene synonyms/obsolete SPAC18G6.16c; SPAC1783.09c
Gene product transcriptional regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain
Entry clone Cloned
ORF length (unspliced) 2934
ORF length (spliced)
Entry clone length 2934
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1327.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGCAACAGCGTTCAGG
Rev primer name SPAC1327.01.Rv
Rev primer SEQ AGAAAGCTGGGTATCCGGCAAACGAAATATTC
Amino acid length 977
Molecular weight 107.8
Isoelectric point (calc.) 6.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 64
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus; cytoplasmic dots
Comments for localization except nucleolus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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