| Gene name |
SPAC31A2.14 |
| Gene ID |
47/F04 |
| Gene synonyms/obsolete |
|
| Gene product |
WD repeat protein;
similar to Sp SPCC16A11.02 (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2966 |
| ORF length (spliced) |
2889 |
| Entry clone length |
2966 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC31A2.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGTTCGGAAACGGTA |
| Rev primer name |
SPAC31A2.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATATTAAGACTCTGGGTT |
| Amino acid length |
962 |
| Molecular weight |
107.2 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; periphery at
site of septum formation and cell tip |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>cytosol ) |
| Microscope used for
observation |
Leica |