| Gene name |
SPAC27D7.01c |
| Gene ID |
47/H09 |
| Gene synonyms/obsolete |
SPAC1851.04c |
| Gene product |
sequence orphan;
hypothetical protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3159 |
| ORF length (spliced) |
|
| Entry clone length |
3159 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC27D7.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTACTTCCTTCATGGAAC |
| Rev primer name |
SPAC27D7.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATTTATCCACTTCTTTC |
| Amino acid length |
1052 |
| Molecular weight |
119.5 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLVLINGKLVL/LPEIVRALLLL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol (intranuclear microtubule bundle?) |
| Microscope used for
observation |
Leica |