Gene name |
SPBC1861.04c |
Gene ID |
48/A12 |
Gene synonyms/obsolete |
|
Gene product |
similar to C-term of
yeast U4/U6 splicing factor; RNA-binding protein; involved in
mRNA splicing; rrm RNA recognition motif |
Entry clone |
Cloned |
ORF length (unspliced) |
3227 |
ORF length (spliced) |
3045 |
Entry clone length |
3227 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1861.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCAAATACAAGATTT |
Rev primer name |
SPBC1861.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTAAAAACATTTTCCTA |
Amino acid length |
1014 |
Molecular weight |
118 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
965 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYPLIPELWL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |