Gene name |
SPCC1322.06 |
Gene ID |
48/B01 |
Gene synonyms/obsolete |
|
Gene product |
karyopherin;
importin-beta family; similar to human RAN binding protein 11;
structural constituent of nuclear pore |
Entry clone |
Cloned |
ORF length (unspliced) |
3229 |
ORF length (spliced) |
2952 |
Entry clone length |
3229 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1322.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAGTAGATCCTGTAGT |
Rev primer name |
SPCC1322.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGCATGGATTGAAACTGT |
Amino acid length |
983 |
Molecular weight |
112.9 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLDLLITKLLI/LNSSLLSRLLL |
Localization (YFP) |
nuclear envelope;
nucleus |
Comments for localization |
except nucleolus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |