Gene name |
SPBC776.08c |
Gene ID |
48/B12 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein
Nrap; interacts with condensed chromosomes during mitosis;
involved in ribosome biogenesis and assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
3345 |
ORF length (spliced) |
3294 |
Entry clone length |
3345 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC776.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGGTTTGAAAAGAGA |
Rev primer name |
SPBC776.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCTTTGAGTTGTAACTGG |
Amino acid length |
1097 |
Molecular weight |
126 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPKLISFGLLL |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |