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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23C11.15
Gene ID 48/C01
Gene synonyms/obsolete pst2
Gene product transcriptional regulator; sin3 family corepressor; similar to Sp pst1 (paralog); essential; involved in retroelement propagation (required); involved in transcriptional regulation; involved in chromatin silencing; involved in chromatin assembly/disassembly; acetyltransferase complex; involved in histone deacetylation
Entry clone Cloned
ORF length (unspliced) 3352
ORF length (spliced) 3228
Entry clone length 3352
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC23C11.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACAAACACTAGCGAT
Rev primer name SPAC23C11.15.Rv
Rev primer SEQ AGAAAGCTGGGTATGGGATAAATAGTGCATTA
Amino acid length 1075
Molecular weight 124.8
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LERMPCLTL/LVSGLQAKLCL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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