Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC26H5.01c
Gene ID 48/C02
Gene synonyms/obsolete SPAC23A1.19c
Gene product DEAD/DEAH box helicase
Entry clone Cloned
ORF length (unspliced) 3367
ORF length (spliced) 3192
Entry clone length 3367
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC26H5.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCAAACTCCTATTAA
Rev primer name SPAC26H5.01.Rv
Rev primer SEQ AGAAAGCTGGGTAACAAGAAGGTATTAAAGTT
Amino acid length 1063
Molecular weight 120.9
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDKVGALIL
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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