Gene name |
SPBC3F6.05 |
Gene ID |
48/C12 |
Gene synonyms/obsolete |
rga1 |
Gene product |
GTPase activator;
RhoGAP domain; LIM domain; involved in actin cytoskeletal
organization; involved in actin cortical patch distribution;
involved in cellular morphogenesis; involved in cell wall
biosynthesis; involved in septum formation; involved in
septation; putative GAP for Rho1p |
Entry clone |
Cloned |
ORF length (unspliced) |
3453 |
ORF length (spliced) |
|
Entry clone length |
3453 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC3F6.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCCAAAGGGACGCCAA |
Rev primer name |
SPBC3F6.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGAATCCCTAATGACTGCA |
Amino acid length |
1150 |
Molecular weight |
130.6 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTGLAHYLKL/LSQYIYLLRI/LSDLSNLEL/LTFKLFGLFI |
Localization (YFP) |
periphery at cell tip
and site of septum formation; nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |