| Gene name |
SPACUNK4.07c |
| Gene ID |
48/E05 |
| Gene synonyms/obsolete |
cta4; sev4;
SPAPYUK71.01 |
| Gene product |
P-type ATPase; P4
type; involved in regulation of calcium homeostasis; involved
in cytokinesis; involved in cellular morphogenesis; involved
in microtubule based movement |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3636 |
| ORF length (spliced) |
|
| Entry clone length |
3636 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPACUNK4.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGAGTAAGGCTTTAAT |
| Rev primer name |
SPACUNK4.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTACGAAGAACAATTTCC |
| Amino acid length |
1211 |
| Molecular weight |
136.2 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVQMHKILAL/LNTAIYLLQL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |