Gene name |
SPBC1105.10 |
Gene ID |
48/F07 |
Gene synonyms/obsolete |
|
Gene product |
involved in regulation
of (H+)-ATPase in vacuolar membrane |
Entry clone |
Cloned |
ORF length (unspliced) |
4009 |
ORF length (spliced) |
3894 |
Entry clone length |
4009 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
3806G:A |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1105.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCTGGCATTCACAAA |
Rev primer name |
SPBC1105.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCGAAGCTGAACGCAGAT |
Amino acid length |
1297 |
Molecular weight |
148.7 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMHLSCFLNL/LLFTFECLYI/LGCDLIALGL |
Localization (YFP) |
cytosol=nucleus;
filamentous structure |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |