Gene name |
SPAC1D4.14 |
Gene ID |
48/G10 |
Gene synonyms/obsolete |
SPAC22F3.14c |
Gene product |
THO complex component;
involved in mRNA export; involved in transcription by RNA
polymerase II |
Entry clone |
Cloned |
ORF length (unspliced) |
4942 |
ORF length (spliced) |
4887 |
Entry clone length |
4942 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1D4.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTCGTTGCCAGAAAA |
Rev primer name |
SPAC1D4.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGAATTTTTCTTCTTTTC |
Amino acid length |
1628 |
Molecular weight |
188.8 |
Isoelectric point (calc.) |
8.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |