Gene name |
SPAC27F1.01c |
Gene ID |
48/H03 |
Gene synonyms/obsolete |
SPAC25G10.09c |
Gene product |
actin cortical patch
component; predicted coiled-coil region; EF hand motif;
calcium binding protein; involved in actin cytoskeletal
organization; involved in endocytosis; divergent
repeat-containing; involved in actin cortical patch assembly;
WH2 motif; similar to Sp SPBC800.10C and SPBC83.01; target of
Ark1/Prk1 family kinases (potential) |
Entry clone |
Cloned |
ORF length (unspliced) |
5385 |
ORF length (spliced) |
|
Entry clone length |
5385 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC27F1.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATATCCAAATCAGAT |
Rev primer name |
SPAC27F1.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGACAAATTCCCATACCAA |
Amino acid length |
1794 |
Molecular weight |
193.2 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cell tip and site of
septum formation; cytosol |
Comments for localization |
a lot of peripheral
dots by over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |