Gene name |
SPBC30D10.10c |
Gene ID |
48/H11 |
Gene synonyms/obsolete |
tor1 |
Gene product |
phosphatidylinositol
kinase related kinase; TOR signaling pathway; involved in
starvation response (required); involved in stress response;
similar to Sp SPBC216.07c |
Entry clone |
Cloned |
ORF length (unspliced) |
7008 |
ORF length (spliced) |
|
Entry clone length |
7008 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC30D10.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTATTTTAGTGATCT |
Rev primer name |
SPBC30D10.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCAAAAACTACACCATCCT |
Amino acid length |
2335 |
Molecular weight |
266.1 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEKLLTLGI/LQDILRLLNL/LPRIKHLEL |
Localization (YFP) |
cytosol; cytoplasmic
dots |
Comments for localization |
cytoplasmic dots by
over expression? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica;
DeltaVision |