Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H5.04
Gene ID 49/A01
Gene synonyms/obsolete aar2
Gene product U1 snRNP component
Entry clone Cloned
ORF length (unspliced) 1041
ORF length (spliced)
Entry clone length 1041
No. of intron 0
Sequence status Finished
Sequence results 407C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3H5.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTATAGAGTTTGTCGG
Rev primer name SPAC3H5.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGAATTGGATCTTCTTCA
Amino acid length 346
Molecular weight 39.2
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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