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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC977.17
Gene ID 49/C06
Gene synonyms/obsolete
Gene product putative transmembrane channel protein; MIP water channel
Entry clone Cloned
ORF length (unspliced) 1797
ORF length (spliced)
Entry clone length 1797
No. of intron 0
Sequence status Finished
Sequence results 674A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC977.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGTCCCTTTACGCTT
Rev primer name SPAC977.17.Rv
Rev primer SEQ AGAAAGCTGGGTAATTATTCTCAATCGGAGAA
Amino acid length 598
Molecular weight 65.8
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain 6
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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