Gene name |
SPAPB1E7.09 |
Gene ID |
49/D10 |
Gene synonyms/obsolete |
|
Gene product |
glycosyl transferase
family 39; dolichyl phosphate-mannose-protein
mannosyltransferase; involved in O-glycosylation; MIR
domains |
Entry clone |
Cloned |
ORF length (unspliced) |
2220 |
ORF length (spliced) |
|
Entry clone length |
2220 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
408T:C / 1778T:C /
1905T:C / 1991T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPB1E7.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTATGAACAGCTTCA |
Rev primer name |
SPAPB1E7.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCATGAACATTCCAAGAG |
Amino acid length |
739 |
Molecular weight |
85 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGYFLVPLLL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |