Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP7E8.02
Gene ID 49/D12
Gene synonyms/obsolete SPBPB7E8.02
Gene product putative RNA pol II specific subunit C82/hRPC62
Entry clone Cloned
ORF length (unspliced) 2250
ORF length (spliced)
Entry clone length 2250
No. of intron 0
Sequence status Finished
Sequence results 1512T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP7E8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCACTGTTGTAGGTGA
Rev primer name SPBP7E8.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTACAAATTGGTTTGTA
Amino acid length 749
Molecular weight 82.2
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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