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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP22H7.02c
Gene ID 49/E07
Gene synonyms/obsolete pi029; SPACTOKYO_453.05c
Gene product RNA-binding protein; rrm RNA recognition motif; involved in ribosome biogenesis and assembly
Entry clone Cloned
ORF length (unspliced) 2502
ORF length (spliced)
Entry clone length 2502
No. of intron 0
Sequence status Finished
Sequence results 761G:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP22H7.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGCGTATAATTATAAA
Rev primer name SPBP22H7.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTGTCTCAATAAGACGC
Amino acid length 833
Molecular weight 93.6
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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