Gene name |
SPAC1093.01 |
Gene ID |
49/G11 |
Gene synonyms/obsolete |
SPAC12B10.18 |
Gene product |
PPR domains;
translational regulator activity |
Entry clone |
Cloned |
ORF length (unspliced) |
3786 |
ORF length (spliced) |
|
Entry clone length |
3786 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
946T:G / 948T:C /
3722A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1093.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTTACACGAAAGCTTG |
Rev primer name |
SPAC1093.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTGATAGAACTATTAATT |
Amino acid length |
1261 |
Molecular weight |
141.9 |
Isoelectric point (calc.) |
7.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
mitochondrion? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |