| Gene name |
SPAC30C2.01c |
| Gene ID |
50/A02 |
| Gene synonyms/obsolete |
dhc1;
SPAC1093.06c |
| Gene product |
dynein heavy chain,
cytosolic; involved in chromosome alignment in meiotic
prophase (required); involved in nuclear migration (sensu
Fungi) (required) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
12591 |
| ORF length (spliced) |
|
| Entry clone length |
12591 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1132G:T /
7108T:C |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC30C2.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAAAAACGATAATTC |
| Rev primer name |
SPAC30C2.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGCACATACTAAAATAA |
| Amino acid length |
4196 |
| Molecular weight |
484.3 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; microtubules;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |