Gene name |
SPAC23G3.02c |
Gene ID |
50/A04 |
Gene synonyms/obsolete |
|
Gene product |
peptide synthetase;
phosphopantetheine attachment site (3); no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
15005 |
ORF length (spliced) |
14775 |
Entry clone length |
15005 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
6564A:addition /
6565C:A / 1069?G:C |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC23G3.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAATTCAAATGAAGA |
Rev primer name |
SPAC23G3.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTTCAATTTCCTTAATA |
Amino acid length |
4924 |
Molecular weight |
559.8 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGRIINGKLYI/LAPSVDSLAI/LFKTLLNNLPI/LKNNLKPLLL/LREIFHLRI/LLDIKKWLNL/LPTAISALKI/LFNTIPFPLFL/LNWLTSLDL/LKDFRENLLL/LQYISLLDL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |