Gene name |
SPAC227.13c |
Gene ID |
50/A08 |
Gene synonyms/obsolete |
isu1 |
Gene product |
iron-sulfur protein;
Nif-u like protein; involved in iron metabolism; complexed
with ferredoxin |
Entry clone |
Cloned |
ORF length (unspliced) |
579 |
ORF length (spliced) |
|
Entry clone length |
579 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC227.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGTCTTCCGTCGCTC |
Rev primer name |
SPAC227.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGGTAGCAGACTCAATA |
Amino acid length |
192 |
Molecular weight |
20.6 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |