| Gene name |
SPAPB15E9.02c |
| Gene ID |
50/B07 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan; has transcript on microarray |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
567 |
| ORF length (spliced) |
|
| Entry clone length |
567 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
#check |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAP15E9.02.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGACAGGAATGTTTTTTTT |
| Rev primer name |
SPAP15E9.02.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAACTACGCCATGCAATGCAG |
| Amino acid length |
188 |
| Molecular weight |
23.2 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
3 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |