Gene name |
SPAPB17E12.03 |
Gene ID |
50/C05 |
Gene synonyms/obsolete |
|
Gene product |
involved in peroxisome
biogenesis |
Entry clone |
Cloned |
ORF length (unspliced) |
1074 |
ORF length (spliced) |
936 |
Entry clone length |
1074 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAPB17E12.03.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGATTCGCCTTCATTATT |
Rev primer name |
SPAPB17E12.03.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAAATCATTAGTCTCCAAAAG |
Amino acid length |
311 |
Molecular weight |
35.9 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
as bright dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |