Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB2B2.11
Gene ID 50/C06
Gene synonyms/obsolete
Gene product putative dTDP-glucose 4,6-dehydratase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1098
ORF length (spliced)
Entry clone length 1098
No. of intron 0
Sequence status Finished
Sequence results 580A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPB2B2.11.Fd
Fwd primer SEQ GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGACTGGGGACTATAAAGA
Rev primer name SPBPB2B2.11.Rv
Rev primer SEQ GGGGACCACTTTGTACAAGAAAGCTGGGTAATAGTACTCGTCAATTAAT
Amino acid length 365
Molecular weight 42.6
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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