Gene name |
SPAC12B10.09 |
Gene ID |
50/E12 |
Gene synonyms/obsolete |
|
Gene product |
mitochondrial carrier;
involved in mitochondrial transport; unknown specificity
|
Entry clone |
Cloned |
ORF length (unspliced) |
1325 |
ORF length (spliced) |
1038 |
Entry clone length |
1325 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC12B10.09.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGATTTTTAACTATGTTTT |
Rev primer name |
SPAC12B10.09.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTACAATCCTTCGGCTTTCATA |
Amino acid length |
345 |
Molecular weight |
38.4 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
5 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYWDVFSKLII |
Localization (YFP) |
no apparent signal
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
DeltaVision |